Table 2.

Comparisons of genome editing methods assisted by CRISPR-Cas system.

Comparison	Method	This study	Cas9-assisted method (Li et al., 2015)	Cas12a-assisted method (Yan et al., 2017)	NO SCAR (Reisch and Prather, 2015)
Component	Cas gene	Codon-optimized FnCas12a	SpCas9	FnCas12a	SpCas9
	Donor DNA	Circular (plasmids)	Linear (PCR products)	Linear (PCR products or oligonucleotides)	Linear (PCR products or oligonucleotides)
Editing efficiency	At a single locus	Nearly 100%	Nearly 100%	More than 50%	85 ∼ 100%
	At multiple loci	About 20% for 3 gene insertions	About 20% for 3 point mutations	Not tested	Not tested
Time	1 mutation	8 days	7 days	7 days	8 days
	2 mutations	8 days	13 days	13 days	11 days
	3 mutations	8 days	19 days	19 days	14 days