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. 2018 Oct 9;9:1026. doi: 10.3389/fphar.2018.01026

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Copyright © 2018 Gu, Wu, Su, Xing, Shen, He, Li, Yuan, Tang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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E2 treatment accelerated myelin formation in DRG neurons co-culture with Schwann cells. (A–D) Schwann cell/DRG neuron cocultures were grown in myelin-promoting media with or without E2 and maintained for 28 days, the cocultures were photographed (A) or fixed and stained for MAG (B), red arrow showing the myelinated axons, scale bar, 50 μm. (C) The numbers of myelin sheaths per high-powered field were counted. The ratio of mean values for each E2 and vehicle from three representative experiments are shown. The number of myelin sheaths in the cultures treated with E2 was significantly higher than that in control cultures. ^∗∗p < 0.01, Student’s t-test, n = 3 cultures per group. All the data are mean ± SEM. (D) Western blot for the expressions of myelin proteins MAG, β-actin served as a protein loading control. The results showed the expression of MAG in cocultures with E2 treatment was higher than that without E2.