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. 2018 Oct 9;9:1026. doi: 10.3389/fphar.2018.01026

FIGURE 2.

FIGURE 2

Schwann cell proliferation was not modulated by E2. Schwann cells cultured on fasciculated DRG axons (axonal membrane) or PDL with or without E2 for 24 h, and the cell proliferation was measured by using EdU labeling. (A) The images of EdU staining (red) and Hoechst 33342 staining (blue) were used to measure cell proliferation, scale bar, 50 μm. Cell counting was performed in at least 10 random microscopic fields (200× magnification). (B) Histograms showing that there were no significant changes in cell proliferation of SCs between E2 treatment or not in cultured on fasciculated DRG axons or PDL. P > 0.05, Student’s t-test, n = 3 cultures per group. All the data are mean ± SEM.