FIGURE 4.

E2 treatment accelerated Schwann cell differentiation via the estrogen receptors (ERs). (A) Immunocytochemistry with anti-MAG (green) displayed the SC differentiation efficacy of the different inducers that were cultured in vehicle or with E2 treatment, or ICI182780 (antagonist of ERs) treatment, or E2 plus ICI182780 treatment. Scale bar, 50 μm. (B) Western blotting compared the MAG expression in differentiated cells treated with E2, ICI182780, E2 plus ICI182780 for 1, 2, and 3 days, and vehicle as control. Also shown (Upper) are representative Western blot images, in which β-actin served as an internal standard. ^∗∗p < 0.01 versus vehicle, and ^##p < 0.05 versus E2. One-way ANOVA, n = 3 cultures per group. All the data are mean ± SEM.