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. 2018 Oct 9;9:2198. doi: 10.3389/fimmu.2018.02198

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Copyright © 2018 Jog, Chakravarty, Guthridge and James.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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vIL-10 induces limited STAT3 phosphorylation in monocytes. Monocytes were enriched from PBMCs using magnetic beads and stimulated with 10 ng/ml hIL-10 or vIL-10 for 30 min. pSTAT3 (pY705) was measured by flow cytometry. (A) Representative histogram for pSTAT3. (B) Mean fluorescence intensities for pSTAT3 are shown as fold increase over unstimulated. Data are presented as mean ± SD, n = 13, ^***p < 0.001 by Wilcoxon test. (C) Western blot analysis shows increased STAT3 phosphorylation in cells stimulated with hIL-10 compared to those stimulated with vIL-10. Cells from three independent donors were used. Upper panel: phosphorylated-STAT3, lower panel: total STAT3. (D) pSTAT3 presented as ratio of band intensity of pSTAT3 and STAT3 from (C) for each donor. ^*p < 0.05, ^**p < 0.01 by ANOVA.