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. 2018 Oct 15;38(21):e00249-18. doi: 10.1128/MCB.00249-18

FIG 5.

FIG 5

PCSK3, -5, and -6 license GDF15 maturation in vivo. (A) Cardiac Gdf15, Pcsk3, Pcsk5, and Pcsk6 mRNA levels in mice receiving AAV9 pericardial injection. (B) Representative Western blot images of pro-GDF15 and mature GDF15 (framed) in AAV-injected heart lysates. −, negative control (GDF15 KO heart lysate); +, positive control (heart lysates from cardiac ERRα/γ KO mice). β-Actin served as a loading control. Quantification of mature GDF15 band density (normalized to β-actin) and mature versus pro-GDF15 ratio is shown at the bottom. (C) Plasma GDF15 levels in AAV9-injected mice (n = 12 to 18 per group). #, P < 0.0001 versus AAV9-GFP; *, P < 0.01 versus AAV9-GDF15.