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. 2018 Oct 9;9:2089. doi: 10.3389/fimmu.2018.02089

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Copyright © 2018 Hao, Li, Wang, Ma, Zhao, Ye, He, Yang, Gao, Xiao and Wei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of EOGT knockout on Treg cell proliferation and differentiation. Flow cytometry for sorting naive CD4⁺ T cells (CD4⁺CD45RC⁺) (A,D) and CD4⁺CD25⁺ Tregs (A,B) from rat spleens; (C) CD4⁺CD25⁺ Tregs from Eogt^+/+ and Eogt^−/− rats treated with IL-2 without or with different Con A concentrations for 48 h; (E) Foxp3 mRNA levels and (F) percentages of regulatory T cells (CD25⁺Foxp3⁺) differentiated from naive CD4⁺ T cells (CD4⁺CD45RC⁺). Data are mean ± SD from three independent experiments. ^*P < 0.05; ^***p < 0.001, vs. WT control group.