FIG 10.

Treatment with 1E7-03 reduces transcription by promoting binding of phosphorylated VP30 with NP and VP35. (A) Effects of 1E7-03 treatment on minigenome activity. The luciferase signal was normalized to activity in nontreated cells (100%). (B to E) Coimmunoprecipitation of Flag VP30 WT with NP or HA-VP35, as indicated. Western blot analysis with anti-NP and anti-Flag antibodies (green) (B) and with anti-HA (green) or anti-Flag (red) antibodies (D) was visualized with a Li-Cor Odyssey imaging system. Quantification of immunoprecipitated Flag-VP30 and NP and Flag-VP30 and HA-VP35 from the experiments shown in panels B and D, respectively, was performed in Image Studio Lite software. Precipitation of VP30 WT by NP/HA-VP35 was set to 100%. Values are means ± standard errors of the means based on duplicate samples. *, P < 0.05 for results compared to those with the DMSO control (one-way analysis of variance and Dunnett's test). The experiment shown in panel A was performed three times, and the experiments shown in panels B to E were performed at least two times, resulting in essentially similar data.