FIG 3.

Comparison of MARV VP30 (GenBank accession number P35258) with Ravn virus VP30 (RAVV) (Q1PDC6), EBOV VP30 (EBOV) (Q05323), human respiratory syncytial virus M2-1 (HRSV) (P04545), and human metapneumovirus M2-1 (HMPV) (Q8QN58) proteins. Multiple-sequence alignment was done using Clustal Omega. The blue box in the top group of sequences indicates a region rich in arginines and lysines unique for filoviruses, which supports the role of this protein as a transcription antitermination factor. The red box in the second group indicates a zinc finger motif, and the blue box in this group indicates another region rich in arginines and lysines, followed by a leucine-rich region which indicates its role as an oligomerization domain. Note that two leucines are also conserved in RSV and HMPV. The common core roughly corresponds to residues 132 to 253 of MARV VP30. The symbols indicate various levels of sequence conservation: asterisk, fully conserved; semicolon, conserved between groups of amino acids with strongly similar properties; period, conserved between groups of amino acids with weak similarity. Dashes indicate gaps in sequence alignment. The phosphorylated residues identified in the current study are shown in brown.