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Characterization of Q187K188-del mutants. (A) IFA of naive BHK-21 cells transfected with WT or Q187K188-del genome-length RNA. (B) IFA of naive BHK-21 cells infected with the culture fluids harvested on day 4 p.t. from the transfected cells in panel A. (C) Plaque assay. Culture fluids from the transfected BHK-21 cells on day 5 p.t. were subjected to plaque assay using BHK-21 cells. No detectable (N.D.) plaques were observed for the Q187K188-del mutant.
