Skip to main content
. 2018 Oct 12;92(21):e01002-18. doi: 10.1128/JVI.01002-18

FIG 9.

FIG 9

Characterization of PIVNS2A replication. (A to D) Electron microscopic analysis of naive and NS2A-expressing BHK-21 cells infected with WT DENV-2 and PIVNS2A. (A) Naive BHK-21 cells infected with WT virus at 48 h postinfection. (B) Naive BHK-21 cells infected with PIVNS2A P30 (selection I) at 48 h postinfection. (C) NS2A-expressing BHK-21 cells infected with WT virus at 48 h postinfection. (D) NS2A-expressing BHK-21 cells infected with PIVNS2A P30 (selection I) at 72 h. Scale bars, 200 nm. The arrowheads indicate virus particles. VP, vesicle packets. (E and F) Growth kinetics of the WT, selection I recombinant PIVNS2A (K188-del plus G100VprM plus P179SNS2A plus A373VNS3), and selection II recombinant PIVNS2A (K188-del plus T69IE plus I144TNS2B plus E477DNS3) on NS2A-expressing BHK-21 cells at MOI of 0.1 (E) and 1 (F).