FIG 3.
K111 mutations do not enable transcriptional activity. C33a cells were either transiently transfected with 50 ng pC18-Sp1-luc (A) or 50 ng pGL CRPV (PL-PE7) firefly reporter construct (B), 10 ng of the empty expression vector (pSG5), or the expression vectors for the different CRPV E2 proteins. A schematic presentation of the reporter constructs is shown above the respective graphs. The values represent the relative luciferase activities of the E2 proteins (wild type or mutants) to the basal activity of the used firefly reporter construct. Error bars indicate the standard error of the mean (SEM) from at least six independent experiments.