FIG 9.
Functional analysis of E1 and E2 mutant proteins. C33a cells were transiently transfected with 50 ng pGL CRPV(PL-PE7) firefly reporter construct, 100 ng E1 expression vectors, and 10 ng E2 expression vectors (as indicated). Empty expression vector (pSG5) was used to adjust differences in DNA amounts. (A) The values represent the relative luciferase activities of either E1, E2, or E1/E2 to the basal activity of the used firefly reporter construct. Error bars indicate the standard errors of the means (SEM) from at least six independent experiments. (B) Total DNA was extracted and digested with DpnI. The extent of DNA replication of the pGL CRPV (PL-PE7) plasmid was determined by qPCR. Data are shown relative to the extent of replication in the presence of the empty vector (vector). Error bars indicate the SEM.