FIG 4.

Normal expansion and activation of adoptively transferred TCR transgenic T cells after acute HBV transduction under reduced T cell homing to lymphoid organs. (A) Absolute numbers of adoptively transferred COR93-specific CD8⁺ T cells in the livers, lymph nodes, and spleens of the B6 mice that received saline plus sham surgery (white) or anti-CD62L antibodies (αCD62L) and splenectomy (black). Splenectomy and sham surgery were performed at least 1 month before the experiment. αCD62L was administered on days 1 and 2 after adoptive transfer and every 3 days thereafter. On the next day after adoptive transfer, the mice were hydrodynamically transfected with 1.3-HBV plasmid DNA. The absolute numbers of COR93-specific CD8⁺ T cells were monitored on day 3 after the hydrodynamic transfection. The lymph nodal data represent the total number of CD8⁺ CD45.1 cells in bilateral axillary, inguinal, and mesenteric lymph nodes. (B) Frequency of CD69 expressing cells among the intrahepatic COR93-specific CD8⁺ T cells shown in Fig. 4A. The data represent means ± the SD of three mice.