FIG 6.

Relative contributions of MHC class I expression on hematopoietic cells versus nonhematopoietic cells to the induction of HBV-specific CD8⁺ T cells after HBV transduction. (A to C) Six to ten weeks after bone marrow transplantation, the reconstitution efficiency of bone marrow chimeric mice was evaluated by measuring the number of total and H-2Kb-expressing lymphocytes (A), I-A/I-E⁺ cells (mostly pAPCs) (B), and CD11b⁺ cells (mostly Kupffer cells, myeloid DCs, and neutrophils) (C). (D) The number of adoptively transferred COR93-specific CD8⁺ T cells in the liver of B6 mice in which MHC class I expression is mostly restricted to nonhematopoietic cells (red bars) or hematopoietic cells (blue bars) was analyzed on day 7 after hydrodynamic transfection with 1.3-HBV plasmid DNA. The number of COR93-specific T cells transferred to control B6 mice reconstituted with B6 bone marrow is shown in white. The numbers of COR93-specific CD8 T cells in the recipient mice (Pre) were analyzed before adoptive transfer of COR93-specific T cells. (E and F) Percentages of CD69-positive and GrB-positive COR93-specific CD8⁺ T cells in the liver, respectively. The data represent means ± the SD for three mice. The fractions of CD69⁺ and granzyme B-expressing donor COR93-specific CD8⁺ T cells (Donor) are also indicated.