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. 2018 Oct 12;92(21):e00813-18. doi: 10.1128/JVI.00813-18

FIG 1.

FIG 1

Human lymphocyte engraftment levels of BRGS mice pre- and postinfection with EBV. To develop an EBV-2 hu-mouse model, BRGS mice transplanted with CD34+ HSCs isolated from human umbilical CB (hu-mice) were used. (A) CD34+ CB-engrafted BRGS mice were analyzed by flow cytometry with antibodies to murine CD45 and to human CD45, CD3, and CD19 prior to infection and at the time of euthanasia to determine human lymphocyte engraftment levels. (B) Prior to infection, blood samples from hu-mice were analyzed by flow cytometry to confirm engraftment of human lymphocytes. Human chimerism was calculated for each mouse, identified by number on the x axis, and is shown as the percentage of human hematopoietic cells in the total population (human and mouse) of hematopoietic cells [(percentage of hCD45 cells/percentage of hCD45 + mCD45 cells) × 100]. (C) Following euthanasia of EBV-infected hu-mice, the spleen, LN, and blood were harvested. A proportion of each sample was used for flow cytometric analysis of human lymphocyte subsets. Human hematopoietic chimerism was calculated as the percentage of human hematopoietic cells in the total population (human and mouse) of hematopoietic cells [(percentage of hCD45 cells/percentage of hCD45 + mCD45 cells) × 100]. Human B cell (hCD45+/hCD19+) and T cell (hCD45+/hCD3+) subsets were calculated as a percentage of total human CD45+ cells.