FIG 5.
Histopathological lesions in wild-type and JH knockout gnotobiotic piglets experimentally infected with HEV. (A) Histopathological lesions in the liver, including lymphoplasmacytic hepatitis and hepatocellular necrosis. Lymphoplasmacytic hepatitis was scored as follows: 0, no inflammation; 1, 1 to 2 focal lymphoplasmacytic infiltrates/10 hepatic lobules; 2, 3 to 5 focal infiltrates/10 hepatic lobules; 3, 6 to 10 focal infiltrates/10 hepatic lobules; and 4, >10 focal infiltrates/10 hepatic lobules. Hepatocellular necrosis was characterized by the presence of individual hepatocytes with an eosinophilic cytoplasm with or without fragmented or absent nuclei. (B) Liver/body weight ratio. The liver weights were evaluated as a ratio of the overall body weight. Asterisks indicate statistical significance as determined by two-way ANOVA (*, P < 0.05; **, P < 0.01; ***, P < 0.001).