FIG 6.
Frequencies of IFN-γ and IL-4 intracellular cytokines in mononuclear cells isolated from spleen and mesenteric lymph node tissues and PBMCs from the peripheral blood of wild-type and JH knockout piglets experimentally infected with HEV. PBMCs and MNCs were collected from each piglet at 28 dpi at necropsy. Cells were gated on CD3 and stained for extracellular and intracellular markers CD4+ and IFN-γ and IL-4, respectively, after stimulation with HEV-specific capsid protein. The mean frequencies of IFN-γ (A), IL-4 (B), and CD4+ (C) are indicated and expressed as means ± the SEM. All frequencies were determined by flow cytometry with 100,000 events per sample (*, P < 0.05).