FIG 7.

Frequencies of TGF-β and IL-10 intracellular cytokines in mononuclear cells isolated from spleen and mesenteric lymph node tissues and PBMCs from the peripheral blood of wild-type and J_H knockout piglets experimentally infected with HEV. PBMCs and MNCs were collected from each piglet at 28 dpi at necropsy. Cells were gated on CD3 and stained for extracellular and intracellular markers CD4⁺, CD25⁺, Foxp3, and TGF-β and IL-10, respectively, after stimulation with HEV-specific capsid protein. The mean frequencies of CD25⁺ Foxp3⁺ (A), CD25⁻ Foxp3⁺ (B), TGF-β (C), and IL-10 (D) are indicated and expressed as means ± the SEM. All frequencies were determined by flow cytometry with 100,000 events per sample.