TABLE 4.
Genotyping the Ig heavy chain modified piglets
| Pig | Mutation | Genotypea | Length (bp) |
|---|---|---|---|
| WT | None | GCACACCCCCCAGGTTTTTGTGGGGCGAGCCTGGAGATTGCACCACTGTGATTACTATGCTATGGATCTCTGGGGCCCAGGCGTTGAAGTCGTCGTGTCCTCAGGTAAGAACGGCCC | |
| 308-1 | Homozygous | GCACACCCCCCAGGTTTTTAGTGGGGCGAGCCT | 1 |
| 308-2 | Biallelic mutation | GCACACCCCCCAGGTTTTTTGTGGGGCGAGCCTGGAG | 1 |
| GCACACCCCCCAGGT - - - - - - GGGGCGAGCCTGGAG | 6 | ||
| 308-3 | Homozygous | GCACACCCCCCAGGT - - - - - - - GTGGGGCGAGCCTGGAG | 4 |
| 68-8-1 | Biallelic mutation | GCACACCCCCCAGGT - - - - GTGGGGCGAGCCTGGAG | 4 |
| GCACACCCCCCAGGTT - - - - | 282 | ||
| 68-8-2 | Homozygous | GCACACCCCCCAGGT - - - - GTGGGGCGAGCCTGGAG | 4 |
| 68-8-3 | Biallelic mutation | GCACACCCCCCAGGTTTTTTTTGTGGGGCGAGCCTGGAG | 3 |
| GCACACCCCCCAGGTTTTTTGTGGGGCGAGCCTGGAG | 1 | ||
| 68-8-4 | Biallelic mutation | GCACACCCCCCAGG - - - - - GTGGGGCGAGCCTGGAG | 5 |
| GCACACCCCCCAGGTTTTTTGTGGGGCGAGCCTGGAG | 1 | ||
| 68-8-5 | Biallelic mutation | GCACACCCCCCAGGTTTTTTGTGGGGCGAGCCTGGAG | 1 |
| GCACACCCCCCAGG - - - - - - GGCGAGCCTGGAGATTGCAC | 9 | ||
| 61-12-1 | Homozygous | GCACACCCCCCAGGCCACACTCACCCCTCCAGGGGCGAGCCTGGAG | 14 |
| 61-12-2 | Homozygous | TCCCAGCCCTGCGGCCGAGGG - - - - - - GTGGGGCGAGCCTGGAG | 59 |
| 61-12-3 | Homozygous | GCACACCCCCCAGG - - - - - - GGGGGCGAGCCTGGAG | 6, 1 |
| 61-12-4 | Biallelic mutation | GCACACCCCCCAGGTTTTTTGTGGGGCGAGCCTGGAG | 1 |
| GGGAAGTCAGGAGGGAGC - - - - - - AGATTGCACCAC | 107 | ||
| 29-1-1 | Homozygous | GCACACCCCCCAGGT - - - - GTGGGGCGAGCCT | 4 |
| 29-1-2 | Homozygous | GCACACCCCCCA - - - - - - GGGGCGAGCCT | 9 |
| 29-1-3 | Biallelic mutation | GCACACCCCCCA - - - - - - GGGGCGAGCCT | 9 |
| GCACACCCCCCAGGTTTTTGTGGGGGCGAGCCT | 1 | ||
| 61-13-1 | Biallelic mutation | GCACACCCCCCAGGTTTTT - - - - - - CTCAGGT | 80 |
| GCACACCCCCCAG - - - - - - GTGGGGCGAGCCTGGAGATTGCACCATGTGATTACTATGCTATGGGATCTCTGGGGCCCAGGCGTTGAAGTCGCTCGTGTCCT - - - GTAAGAACGGCCC | 9, 2, 2 | ||
| 61-13-2 | Biallelic mutation | GCACACCCCCCAGGTTTT - - - - - - - TCGTGTCCTCAGGTAAGAA CGGCCC | 74 |
| GCACACCCCC - - - - - - CAGGTAAGAACGGCCC | 91 | ||
| 61-13-3 | Homozygous | GCACACCCCCCAGGTTTTTGT - - - - - - CGTGTCCTCAGGT | 72 |
| 61-13-4 | Homozygous | GCACACCCCCCAGGTTTTT - - - - - - CTCAGGT | 80 |
a
Genotype of each knockout piglet with mutations on each allele individually or on both alleles. The number of base pairs for each indicated sequence is indicated in column 4. Underlining indicates targeting sequence. Boldface letters indicate insertion or change in nucleotides, and a hyphen (“-”) indicates the deletion of a nucleotide. All piglets carried mutation on the Ig heavy chain.