FIG 7.

Direct binding of SE to NF-κB/Sp1. EMSA conditions were as follows: probe alone (lane 1), 100 μg BSA (lane 2), 25 μg intact SE (lane 3), SE treated with 0.25% Triton X-100 to open the membrane (lane 4), SE treated with Triton X-100 and boiled at 95°C for 30 min or treated with 0.5% SDS to denature the SE proteins (lane 5). Samples were incubated with dI-dC cold competitor (1,000×) on ice for 20 min before addition of NF-κB p65 probe (A and B) or Sp1 probe (C and D) and further incubation on ice for 20 min. Samples were separated on a 4% nondenaturing PAGE gel at 4°C protected from light. C denotes NF-κB or Sp1 complexes. The gel images show one representative experiment of three.