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Raw data, cryo-EM reconstruction, and model validation. (A and B) Cryo-EM micrograph of primed ARV particles (A) and Fourier spectrum of a representative micrograph showing the visibility of Thon rings (B). (C) Fourier shell correlation (FSC) curves showing the masked icosahedral reconstruction (red), unmasked reconstruction under D3 symmetry (green), and unmasked reconstruction without applying any symmetry (blue). (D) Model quality validation. CC, cross correlation.
