Fig. 4.

CCA-induced changes in Tom40 assembly in isolated mitochondria of young and aged animals. A: representative autoradiogram of Tom40 precursor protein import in mitochondria isolated from control and stimulated muscle of young and aged animals. Radiolabeled Tom40 was imported into mitochondria for 15 and 90 min. Mitochondria were reisolated with digitonin and loaded on a 5–13% blue native gel. Dashed lines indicate the lanes that have been realigned in a single gel. B and C: summary of the data (n = 7–8) where Tom40 incorporation into intermediate I, intermediate II, and the TOM complex was measured in both young (B) and aged (C) animals. †P < 0.05, 15 vs. 90 min; *P < 0.05, Stim vs. Con.