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. 2018 Oct 9;12:341. doi: 10.3389/fncel.2018.00341

Figure 1.

Figure 1

Expression of Shank3 in both glutamatergic and GABAergic neurons. (A,B) Detection of Shank3 mRNA in Vglut1/2 mRNA-positive glutamatergic neurons in the prelimbic region of the medial prefrontal cortex (mPFC) in mice (P56) by double-immunofluorescence in situ hybridization. Note that Vglut2 mRNA signals in the mPFC were weaker than those of Vglut1, and that, in the dorsolateral striatum, Vglut1/2 mRNA signals are very weak or absent. Scale bar, 0.5 mm, 20 μm. (C,D) Detection of Shank3 mRNA in Gad1/2-positive GABAergic neurons in the prelimbic region of the mPFC and the dorsolateral region of the striatum of mice (P56) by double-immunofluorescence in situ hybridization. Scale bar, 0.5 mm, 20 μm. (E,F) Detection of Shank3 proteins in GAD67-positive GABAergic neurons in cultured rat hippocampal neurons at 15 days in vitro (DIV 15), as shown by double immunofluorescence staining for Shank3 and GAD67 (encoded by Gad1). Scale bar, 50 μm, 20 μm, 50 μm, 5 μm.