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. 2018 Oct 9;12:341. doi: 10.3389/fncel.2018.00341

Figure 5.

Figure 5

Strongly altered repetitive behaviors in global Shank3Δ14–16 mice are partially mimicked by Viaat-Cre;Shank3Δ14–16 mice. (A) Enhanced self-grooming in Shank3Δ14–16 mice (11–14 weeks) in a novel cage without bedding. n = 22 (WT) and 21 (KO), ***P < 0.001, Student’s t-test. (B) Enhanced self-grooming and suppressed digging in Shank3Δ14–16 mice (12–14 weeks) in home cages with bedding. n = 21 (WT) and 20 (KO), ***P < 0.001, Mann-Whitney U test. (C) Enhanced self-grooming in Shank3Δ14–16 mice (9–14 weeks) in Laboras cages, in which mouse movements are monitored for 4 days. n = 20 (WT) and 21 (KO), *P < 0.05, **P < 0.01, ***P < 0.001, repeated measures two-way ANOVA (genotype p value = 0.0149 and genotype × time p value < 0.0001). (D) Enhanced self-grooming and suppressed climbing in Shank3Δ14–16 mice (9–14 weeks) in Laboras cages (long-term monitoring). These graphs are a summary of all types of movements in Laboras cages for 4 days, including self-grooming. n = 20 (WT) and 21 (KO), *P < 0.05, ***P < 0.001, ns, not significant, Student’s t-test. (E–H) Viaat-Cre;Shank3Δ14–16 mice (11–15 weeks for E, 10–26 weeks (cohort 3: 10–20 weeks, cohort 4: 23–26 weeks) for F, 12–14 weeks for G,H) show enhanced self-grooming and suppressed digging in home cages with bedding (F) but normal self-grooming in a novel cage without bedding (E) and normal self-grooming but enhanced rearing and suppressed climbing in Laboras cages (long-term monitoring; G,H). n = 18 mice (WT), 14 (cKO) for w/o bedding, 14 (WT), 18 (cKO) for w/bedding, and 15 (WT), 13 (cKO) for Laboras, *P < 0.05, **P < 0.01, ***P < 0.001, repeated measures two-way ANOVA (for Laboras; genotype p value = 0.79), Student’s t-test (for self-grooming test, repetitive behavior test, and Laboras (climbing, rearing, grooming and drinking)), and Mann-Whitney U test (for Laboras (eating)).