FIGURE 2.
Olfactory sensory neuron (OSN) responses after stimulation with odorants used in the DREAM technique. (A) Schematic drawing of Single Sensillum Recording (SSR) procedure. (Bi-Biv) Representative SSR traces of sensilla activity upon presentation of the respective solvents, an inhibitory interaction (guaiacol 10−1, ab5A), no interaction (valencene 10−4, ab6B), an excitatory interaction (3-methylthio-1-propanol 10−4, ac3B), and a highly excitatory interaction (methyl butyrate 10−1, ab3A). The black bar marks stimulus delivery and duration (0.5 s). Colored boxes correspond to heat map in (C). The OSN response after subtraction of possible solvent responses is stated on the bottom of each colored box. (C) Color-coded average responses i.e., frequency of action potentials (AP) measured in SSRs to stimulation with odorants at a 10−4 and a 10−1 dilution (n ≥ 4). Changes in spontaneous chemosensory receptor activity induced by used solvents have been subtracted from the recorded firing frequency. Numerical values can be found in Supplementary Table S2. Blue diagonal line represents expected, ideal results based on original publication (von der Weid et al., 2015).