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Post mortem human brain tissue block (Case 1) at different stages of clearing process. 8 mm thick sample from temporal lobe prior to clearing (a), after 7 months of CLARITY (b), after 10 months of CLARITY and optical index matching in TDE (c). (d) Part of the block (indicated with white frame in (a)) after 14 months of CLARITY and refractive index matching on the title page of the Werner Spalteholz’ publication on the first tissue clearing method (Spalteholz, 1914).
