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Immunohistochemical staining efficiency of 3 mm thick subsample (1I). (a) Reconstruction of a fast z-line scan over the cortical profile with selective stacks (z = 100 μm) of upper (b), middle (c) and deeper (d) layers of a 3 mm thick cortex tissue block. Neurons (HuC/D, green) and astrocytes (GFAP, cyan) are visible throughout the block with slight variations in staining intensity. Upper stack (b) shows fibers of cortical layer I.
