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. 2018 Jul 5;64(5):393–400. doi: 10.1262/jrd.2018-033

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This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 1. — Visualization of ram sperm DNA damage using the sperm chromatin dispersion (SCD) test (a, b) and a 2T-Comet Assay (c). Low (a) and high (b) levels of sperm DNA fragmentation in two different rams using the sperm chromatin dispersion test. Red arrows: nucleoids with fragmented DNA. White arrows: nucleoids containing non-fragmented DNA. (c) 2-Tails comet assay showing electrophoretic fields mapping the different SSBs and DSBs positioning. Scale bars represent 10 µm.