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. 2019 Jan;153:105–113. doi: 10.1016/j.pep.2018.09.001

Fig. 4.

Fig. 4

ESI-MS analyses of intact hsEH CTD and FL. A.m/z detection of intact hsEH CTD. The m/z spectrum showed a Gaussian-type distribution of multiply charged ions ranging from m/z 1275.2 to 2475.7. B. Deconvolution of intact hsEH CTD m/z spectrum. The mass of the recombinant hsEH CTD was 39497.9 Da, virtually identical to the one computed with ProtParam. C.m/z detection of intact hsEH FL. The m/z spectrum showed a Gaussian-type distribution of multiply charged ions ranging from m/z 1196.3 to 2289.7. D. Deconvolution of intact hsEH FL m/z spectrum. The deconvoluted mass of the recombinant hsEH FL was 67582.1 Da, corresponding to the molecular weight of the protein upon N-terminal methionine excision.