Figure 2.

Spdef is required for mucous cell differentiation and Muc5b expression in tracheal submucosal gland and superficial epithelium. Representative micrographs illustrating mucous cell differentiation (A) hematoxylin and eosin (H&E) staining, (B) glycoconjugates (AB-PAS staining), (C and D) Muc5b (submucosal glands and superficial epithelium, respectively), and (E) Muc5ac immunohistochemistry in PND 42 Spdef^−/− and wild-type littermates. Micrographs are montages acquired with a slide scanner as described in the Methods section of the text. Scale bars: 20 μm (A–C and E) and 200 μm (D).