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. 2018 Sep;59(3):383–396. doi: 10.1165/rcmb.2017-0127OC

Figure 4.

Figure 4.

Loss of Spdef improves early survival but fails to decrease airway mucus obstruction in neonatal Scnn1b-transgenic (Scnn1b-Tg) mice. (A) Survival curves for the progeny of the Spdef−/− × Scnn1b-Tg cross. n = number of mice per genotype. *P < 0.05 versus Spdef-sufficient (Spdef+/+) mice, wild-type (WT) mice by log-rank test. Dashed line indicates survival of mice for which genotypes were not available owing to early death and cannibalization. (B) Representative micrographs illustrating AB-PAS staining in neonatal (PND 10) Scnn1b-Tg mice that were either Spdef+/+; Scnn1b-Tg or Spdef deficient (Spdef−/−; Scnn1b-Tg). Scale bar: 200 μm. *Airway lumen obstructed by mucus plugs. Insets show high-magnification view of areas highlighted by the arrows in the low-magnification panels. Micrographs are representative of at least three mice for each genotype and are montages acquired with a slide scanner, as described in the Methods section of the text. (C) Quantitative RT-PCR results for Muc5b and Muc5ac transcripts in neonatal (PND 10) Scnn1b-Tg mice that were either Spdef+/+; Scnn1b-Tg (open diamond) or Spdef−/−; Scnn1b-Tg (solid diamond) (n = 5–6/genotype).