Loss of Spdef increases airway neutrophil infiltration in Scnn1b-Tg mice. (A–D) Differential BAL cell counts: (A) neutrophils, (B) macrophages, (C) eosinophils, and (D) lymphocytes in neonatal (PND 7–10; left panel) or adult (PND 120–170; right panel) mice from the Spdef+/− × Scnn1b-Tg cross: Spdef-sufficient (Spdef+/+; open dots), Spdef-deficient (Spdef−/−; solid dots), Spdef-sufficient Scnn1b-Tg (Spdef+/+; Scnn1b-Tg open diamonds), or Spdef-deficient Scnn1b-Tg (Spdef−/−; Scnn1b-Tg solid diamonds). *P < 0.05 versus Spdef+/+; Scnn1b-Tg mice by one way ANOVA. BALF = BAL fluid.