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High-fat diets increased the levels of serotonin in small intestine. (a) Serotonin staining of small intestine in the three groups (×4). (b), (c) Brown areas show serotonin positive (×40). (d) The areas of serotonin positive in small intestine were measured in randomly selected fields from each slide. Statistical graph of quantified optical density is shown in (d). ∗p=0.012, compared with the control diet-fed rats.
