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Quantification of cysteines present in COS using the Ellman's reaction. Values are the average of three independent determinations with their corresponding standard deviations.
| Treatment of protein sample | Number of detected Cys per mole of protein |
|---|---|
| None (native protein) | 0.30±0.01 |
| Urea 8 M | 3.99±0.03 |
| Urea 8 M+DTT | 6.05±0.09 |
| Urea 8 M+TCEP | 5.98±0.01 |
