Figure 1.

Schematic and workflow of centrifuge droplet generation. (a) Illustration of microfluidic cross-flow PDMS droplet generator with 25 µm by 25 µm nozzle dimensions on a plastic device holder; (b) A photograph of the actual plastic holder with the PDMS droplet generator, syringe reservoirs, tubing, and collection tube fully installed; (c) The PDMS droplet generator (i) is installed on the plastic holder; (d) A pipette tip is used to load the aqueous (ii) and oil (iii) into the syringe reservoir; (e) To generate droplets, the device is loaded into a standard 50 mL tube and spun in a centrifuge; (f,g) During centrifugation, hydrostatic pressure generated by spinning pumps the fluids through the droplet generator and produces the emulsion (iv).