FIG. 2.

Comparison of the relative affinities of USF2a and TFE3 for MLP and L4 binding sites. (A) Representative EMSA resolving the migrating complexes generated in presence of the radiolabeled MLP oligonucleotide and whole COS cell extracts after transfection with an empty vector (control) or a mix of whole-cell extracts after transfection with the expression vector for USF2a (0.1 μg) or TFE3 (10 μg) (transfected). (B, C) The relative DNA binding affinities of USF2a and TFE3 for MLP and L4 were evaluated by competition in gel shift assays with increasing amounts of unlabeled MPL and L4 oligonucleotides, respectively, as shown on the right. The percentage of residual DNA binding is plotted on the left.