FIG. 3.

Effects of overexpression of the bHLH-LZ proteins on the G1RE. Transfections were performed in COS cells with the (L4)4-54PK/CAT reporter and the indicated amounts of the expression plasmids for the bHLH-LZ factors. The amount of the transfected expression plasmids was determined from the dose-effect experiments represented in Fig. 1. The amounts of TFE3 and SREBP/ ADD1 expression vectors (10 and 0.25 μg) were optimal. Activities are expressed as fold induction over the activity of the reporter cotransfected with the respective amount of insertless vector. Each bar represents the mean ± SD of at least three independent transfections.