clustal analysis of Neisseria pil gene sequences. The accession numbers for each strain are as follows: Neisseria gonorrhoeae MS11 GenBank accession CP003909.1 (pilE = NGFG_01821; pilS1 copy 1 = NGFG_02253 (2122534–2123035), copy 2 = NGFG_02253 (2123072–2123479), copy 3 = NGFG_02487, copy 4 = NGFG_00014 (2124309–2124659), copy 5 = NGFG_00014 (2124696–2125043); pilS2 copy 1 = NGFG_02484, copy 2 = NGFG_02485; pilS5 = NGFG_02405; pilS6 copy 1 = NGFG_02481, copy 3 = NGFG_02482; pilS7 = NGFG_02431); Neisseria gonorrhoeae FA1090 GenBank accession NC_002946.2 (pilE = 2038021–2037383*; pilS1 copy 1 = 2043862–2044354*, copy 2 = 2044392–2044736*, copy 3 = 2044773–2045127*, copy 4 = 2045506–2045842*, copy 5 = 2045843–2046256*; pilS2 copy 1 = 2040723–2041203*, copy 2 = 2041256–2041603*, copy 3 = 2041646–2042026*, copy 4 = 2042470–2042800*, copy 5 = 2042801–2043147*, copy 6 = 2043148–2043522*; pilS3 copy 1 = 2013715–2014201*, copy2 = 2014238–2014567*, copy 3 = 2014626–2015018*; pilS6 NGO2041a copy 1 = 2015343–2015868, copy 2 = 2016302–2016669, copy 3 = 2016707–2017060; pilS7 = 1480693–1481248*); Neisseria gonorrhoeae NCCP 11945 GenBank accession NC_011035.1 [pilE = NGK_RS08820, pilS1 = NGK_RS09875 (1818155–1818627), pilS2 = NGK_RS09875 (1818628–1819032), pilS3 = NGK_RS09875 (1819033–1819327)]; Neisseria meningitidis FAM18 GenBank accession NC_008767.1 (pilE = NMC_RS01135, pilS1 = NMC_RS00015, pilS2 = NMC_RS00020); Neisseria meningitidis 053442 GenBank accession NC_010120.1 (pilE = NMCC_RS00970; pilS1 = NMCC_RS00130; pilS2 = NMCC_RS00125 copy 1 = 19974–20401, copy 2 = 19532–19973 ; pilS3 = NMCC_RS03380; pilS4 = NMCC_RS07650; pilS5 = NMCC_RS07655; pilS6 = NMCC_RS08075; pilS7 = NMCC_RS08080); Neisseria meningitidis MC58 GenBank accession NC_003112.2 (pilE = NMB0018; pilS1 = NMB0019; pilS2 = NMB0020; pilS3 = NMB0021; pilS4 = NMB0022; pilS5 = NMB0023; pilS6 = NMB0024; pilS7 = NMB0025 copy 1 = 23169–23502, copy 2 = 22825–23168; pilS8 = NMB0026); *Not annotated in final assembly. The midgene antisense promoter is indicated by the left arrow; the midgene sense promoter is indicated by the right arrow. The colour-shaded regions highlight the locations of the antisense and sense promoters within the sequences.