Table 4. Oligonucleotides used to generate mutations in pilE promoter regions.

Oligonucleotide	Description	Sequence
12561-MutC2F	Midgene mutation	5′-CCCCCCTCCGACATCAAAGGCAAATATGTGATCAAGGTTGAAGTTAAAAAC-3′
12562-MutC2R	Midgene mutation	5′-GTTTTTAACTTCAACCTTGATCACATATTTGCCTTTGATGTCGGAGGGGGG-3′
12490-P2F	3′ end mutation	5′-AAGGCATCTGATGCCAAATGAGGCAAATTAGGCGGATCCTTTTAAATAAATCAAGCGGTAAGTGATTTTCCA-3′
12491-P2R	3′ end mutation	5′-TGGAAAATCACTTACCGCTTGATTTATTTAAAAGGATCCGCCTAATTTGCCTCATTTGGCATCAGATGCCTT-3′