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. 2016 Jan 1;162(Pt 1):177–190. doi: 10.1099/mic.0.000199

Table 4. Oligonucleotides used to generate mutations in pilE promoter regions.

Oligonucleotide Description Sequence
12561-MutC2F Midgene mutation 5′-CCCCCCTCCGACATCAAAGGCAAATATGTGATCAAGGTTGAAGTTAAAAAC-3′
12562-MutC2R Midgene mutation 5′-GTTTTTAACTTCAACCTTGATCACATATTTGCCTTTGATGTCGGAGGGGGG-3′
12490-P2F 3′ end mutation 5′-AAGGCATCTGATGCCAAATGAGGCAAATTAGGCGGATCCTTTTAAATAAATCAAGCGGTAAGTGATTTTCCA-3′
12491-P2R 3′ end mutation 5′-TGGAAAATCACTTACCGCTTGATTTATTTAAAAGGATCCGCCTAATTTGCCTCATTTGGCATCAGATGCCTT-3′