Figure 3. Propofol inhibited excessive inflammatory responses in septic mice. Renal mRNA levels of CCL-2 (A and B), interleukin (IL)-1β (A and C), and tumor necrosis factor α (TNF-α) (A and D) were measured by RT-qPCR and verified by agarose gel electrophoresis analysis. Protein expression of NF-κB (p65) in the nucleus (Nuc) was measured by western blotting (E). Data are reported as means±SD (n=12 per group). ***P<0.001 compared with the control group; ^#P<0.05 compared with the cecal ligation and puncture (CLP) group (ANOVA).