Figure 5. CCL-2 is a direct target gene of miR-290-5p. Schematic representation of the putative miR-290-5p binding site in the 3′-untranslated regions (3′UTR) of CCL-2 was predicted by the online database (A). Mouse podocytes cells (MPC5) were co-transfected with the wild type (WT) and mutant (MUT) of CCL-2-3′-UTR and miR-290-5p mimics, and luciferase activity (B), mRNA (C), and protein (D) of CCL-2 were measured. After transfection with the WT and MUT of CCL-2-3′-UTR and miR-290-5p inhibitors, luciferase activity (E), mRNA (F), and protein (G) of CCL-2 were measured. Data are reported as means±SD (n=3 per group). *P<0.05 compared with the normal control (NC) group (ANOVA).