Figure 6. Inhibition of miR-290-5p neutralized the protective effect of propofol in lipopolysaccharide (LPS)-treated mouse podocytes (MPC5). The levels of miR-290-5p were measured by RT-qPCR after being transfected with miR-290-5p inhibitors in MPC5 (A). In LPS-treated MPC5 combined with propofol or propofol+miR-290-5p inhibitors, cell viability was measured using the MTS assay (B); apoptosis was analyzed using flow cytometry (C and D); the mRNA (E) and protein (F) expression of CCL-2 were detected by RT-qPCR and western blotting, respectively. Data are reported as the means±SD (n=3 in each group). *P<0.05 compared with control group; &P<0.05 compared with LPS + propofol group.
