Figure 4. . Effect of indomethacin on bacterial burden, tissue prostaglandin E₂ and histological inflammation levels in mice infected with C. difficile strain 630.

C57BL/6 mice treated with an antibiotic cocktail were challenged with C. difficile 630 strain, following treatment with (Indo) according to the experimental design in Figure 3. (A) Mice were harvested by day 4 after infection and cecum histological scores, (B) C. difficile colonization level in cecal content (C) and PGE₂ were measured. The data are presented as means ± SEM. **p < 0.01, compared with uninfected group by Student t-test, two-tailed; n = 5/group.

PGE₂: Prostaglandin E₂; SEM: Standard error of the mean.