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Schematic illustration of polydimethylsiloxane (PDMS)-based microchannel fabrication based on replica positive master created using a milling technique. (a) The positive master is mechanically milled in an acrylic block; (b) The positive micropatterns are inserted into pre-cured PDMS agent; (c) The solidified PDMS block is peeled off the master; (d) The PDMS block is bonded onto a glass plate by O2 plasma treatment.
