Figure 4.

The percentage of SC23 and SC27 retrieved in different conditions considered in the numerical simulations. The stacked histogram in y-axis shows the percentage of SC23 and SC27 streams retrieved at different channel locations (from 0 to 700 μm). The x-axis in each case denotes the distance along the channel width and the grid points at 45, 195, 345, 495 and 645 μm mark the position of the center of each electrode in the channel. (a) The impact of electrode cross-section on cell separation; (b) Impact of electrode height relative to the channel. The purity of the streams increases with the increase in electrode height; (c) Impact of flow rate. 100% pure streams of SC23 cells can be obtained linearly to the electrodes as the flow rate increases. The purity of SC27 decreases at the same time; (d) Impact of cell concentration on the separation efficiency between cell types at 10 μL/min. SC23 cells are trapped at low concentrations and are not received, the array becomes saturated at high cell concentrations and SC23 streams start appearing collinear with the electrodes.