Figure 5.

(a) The width of stream or zone obtained in the case of each electrode such that >98% pure streams of SC27 can be retrieved in the zone. The width of the zone initially increases with the increase in flow rate until it reaches a peak value before it starts decreasing. The circle shape features the widest zone for the flow rate of 10 µL/min followed by the lens and the diamond shape. However, the width of the zone decreases rapidly as the flow rate increases; (b) The width of the streams for obtaining >98% pure SC27 streams at different heights of the cylindrical electrodes as the flow rate varies. The stream width for the case of heights 50% and 100% of the channel are comparable as seen in this figure and are much higher than the case of height 10% of the channel. This reiterates the effect of height of the electrode for focusing. In this case, the stream width decreases with increase in flow rate; (c) The study of concentration of streams was conducted for stream purities of 98% and above with the increase in flow rates. The zone width increases with flow rate and shows a peak at the flow rate of 10 µL/min, after which it decreases. The zone width increases as the concentration of the cells decrease, mostly owing to less contamination of SC27 cells by SC23 cells that are retrieved through the zones.