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. Author manuscript; available in PMC: 2019 Oct 1.

Published in final edited form as: Dev Biol. 2018 Jul 30;442(1):138–154. doi: 10.1016/j.ydbio.2018.07.009

Figure 10. — After 20 days, EMs differentiated into more ramified microglia morphologies in the presence of NSPCs. (A, B, C and D) Confocal images of EMs + ANS4, EMs, BMDMs + ANS4 and BMDMs with TI < 5 displayed rounded and elongated cell forms. (E, F and G) TI > 5, EMs + ANS4 and BMDMs + ANS4 displayed more amoeboid forms, no BMDMs cultured alone were observed with TI > 5. (H, I, J and K) TI > 15, EMs + ANS4 and BMDMs + ANS4 displayed ramified cell forms, but no EMs cultured alone were observed at TI values > 15. (L and M) Only EMs + ANS4 were observed with TI > 35 and they displayed more ramified cell forms. (N) Quantitative analysis of the morphological changes of EMs or BMDMs into microglia as shown in the distribution of the TI values as function of the culture conditions, EMs + ANS4 significantly had higher TI values compared to the other conditions. (O) Density measurements in EMs + ANS4, EMs, BMDMs + ANS4 and BMDMs cultures. The data show a higher density of EMs or BMDMs identified in groups that contained NSPCs indicating that NSPCs may support the survival or proliferation of EMs and BMDMs.

Figure 10. — After 20 days, EMs differentiated into more ramified microglia morphologies in the presence of NSPCs. (A, B, C and D) Confocal images of EMs + ANS4, EMs, BMDMs + ANS4 and BMDMs with TI < 5 displayed rounded and elongated cell forms. (E, F and G) TI > 5, EMs + ANS4 and BMDMs + ANS4 displayed more amoeboid forms, no BMDMs cultured alone were observed with TI > 5. (H, I, J and K) TI > 15, EMs + ANS4 and BMDMs + ANS4 displayed ramified cell forms, but no EMs cultured alone were observed at TI values > 15. (L and M) Only EMs + ANS4 were observed with TI > 35 and they displayed more ramified cell forms. (N) Quantitative analysis of the morphological changes of EMs or BMDMs into microglia as shown in the distribution of the TI values as function of the culture conditions, EMs + ANS4 significantly had higher TI values compared to the other conditions. (O) Density measurements in EMs + ANS4, EMs, BMDMs + ANS4 and BMDMs cultures. The data show a higher density of EMs or BMDMs identified in groups that contained NSPCs indicating that NSPCs may support the survival or proliferation of EMs and BMDMs.