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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Dev Biol. 2018 Jul 30;442(1):138–154. doi: 10.1016/j.ydbio.2018.07.009

Figure 8.

Figure 8.

EMs increased cord length and numbers in a 3D collagen base in vitro system. After 72 hours, the cord strustures formed by HUVECs cultured alone (A), HUVECs co-cultured with BMDMs (B), and HUVECs co-cultured with EMs (C) imunnostained with PECAM-1 and imaged with confocal microscopy. In the EMs/HUVECs and BMDM/HUVECs co-cultures the distribution of the cord lengths over 40 μm (E) were significantly increased compared to HUVECs cultured alone. In EMs/HUVECs co-cultures the numbers of cords over 40 μm were significantly increased compared to HUVECs co-cultured with BMDMs or alone (F).