Skip to main content
. Author manuscript; available in PMC: 2018 Oct 16.
Published in final edited form as: Methods Enzymol. 2016 Feb 1;573:345–362. doi: 10.1016/bs.mie.2015.12.005

Fig. 1.

Fig. 1

SDS-PAGE analysis of expression and purification steps for a GST-fusion methyllysine reader domain, stained with Coomassie Blue. Shown are (1) ladder, (2) cells preinduction, (3) cells postinduction, (4) lysate pellet, (5) lysate supernatant, (6) flowthrough from glutathione column, (7) wash #1, (8) wash #2, (9) wash #3, (10) wash #4, (11) glutathione beads prior to PreScission protease cleavage, (12) elution, (13) glutathione beads after cleavage with PreScission protease, and (14) concentrated elution. For reference, the fusion domain is boxed in lanes 3 and 11 and the cleaved methyllysine reader domain is boxed in 14.